Figure 7
From: Constitutive degradation of IκBα in human T lymphocytes is mediated by calpain
Pretreatment with E64D inhibits calpain-mediated degradation of IκBα in T cell lysates. T cell lysates obtained from young donors were pooled and 50 μg protein was incubated with 0.01 U purified calpain. The mixture was incubated at 37°C for 15 min. At the end of incubation samples were denatured and resolved using SDS-PAGE. IκBα was detected usingspecific antibody and ECL (A). Values obtained from a minimum of 4 donor pairs following densitometric scanning, are presented as mean integrated density units ± S.D. (B). * indicates significant degradation (p < 0.05).