Figure 7
From: The role of the MAPK pathway alterations in GM-CSF modulated human neutrophil apoptosis with aging
Expression of activated caspase-3 in PMN with aging upon treatment with GM-CSF and PD98059 and SB203580 for 18 h A) Neutrophils of young (upper panel) and elderly subjects (lower panel) were cultured in presence of GM-CSF alone or in combination with either the inhibitor PD98059 (i-ERK) or SB203580 (i-p38), for 18 h. Then, neutrophils were lysed and western-blotting experiments were performed to investigate the amount of active caspase-3. With aging no changes were found by any agents used (lower panel) while neutrophils from young donors (upper panel) showed significant modulation by GM-CSF and PD98059. B, Densitometric analyses were performed as described in the Materials and Methods section for the expression of activated caspase-3 in neutrophils of young (white columns) and elderly donors (black columns) with *p < 0.01.