Figure 1

Frequency distributions (histograms) of MFI representing poly(ADP-ribosyl)ation levels in permeabilised Jurkat cells. (A) Untreated cells, no antibodies (Ab) added (negative control); (B) no NAD⁺ and no oligo in the reaction buffer; primary Ab, secondary Ab; (C) no NAD⁺, but oligo in the reaction buffer; primary Ab, secondary Ab; (D) NAD⁺, but no oligo in the reaction buffer; primary Ab, secondary Ab; (E) NAD⁺ and oligo in the reaction buffer; primary Ab, secondary Ab. Note that a shift to the right (FL-1; x-axis) indicates increased levels of poly(ADP-ribosyl)ation. Numerical values next to the histograms represent the mean fluorescence intensity (MFI). M1, intensity range used to determine mean of FL1-H.