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Figure 1 | Immunity & Ageing

Figure 1

From: The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly

Figure 1

An example showing a scattergram for gating the neutrophil population. A scattergram of HE stained neutrophils (e.g. spontaneous ROS production) in the neutrophil-gated region, and the median channel of spontaneous ROS production (MFI-) and fMLP-stimulated ROS production (MFI+). The median channel for each assay was calculated from the following equation: median channel = 255.75 × log (MFI+ - MFI-), where MFI+ and MFI- are the median fluorescence intensities for a fMLP-stimulated neutrophil population and a no-stimulated neutrophil population, respectively.

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