Figure 1
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1742-4933-5-13/MediaObjects/12979_2008_Article_57_Fig1_HTML.jpg)
An example showing a scattergram for gating the neutrophil population. A scattergram of HE stained neutrophils (e.g. spontaneous ROS production) in the neutrophil-gated region, and the median channel of spontaneous ROS production (MFI-) and fMLP-stimulated ROS production (MFI+). The median channel for each assay was calculated from the following equation: median channel = 255.75 × log (MFI+ - MFI-), where MFI+ and MFI- are the median fluorescence intensities for a fMLP-stimulated neutrophil population and a no-stimulated neutrophil population, respectively.