Number of neutrophils isolated from the saliva and blood of patients with Candida -related denture stomatitis (D.S.) and controls. Saliva and blood samples from diseased (closed bars) and control (open bars) elderly and young groups were obtained as described in the Material and Methods section. The salivary (A) and blood neutrophils (B) were evidenced through staining with Türk's solution and the collected total number determined after counting in a Neubauer chamber. The results are expressed as the mean ± SEM for elderly (n = 14) and young (n = 14) patients or aged (n = 14) and young (n = 14) control subjects tested individually. The data were analyzed by one-way ANOVA followed by Tukey's test and the value of P was considered significant when < 0.01. Equal symbols indicate significant difference between the groups.