Apoptosis of salivary and blood neutrophils from patients with Candida -related denture stomatitis and controls. Salivary (A) and blood neutrophils (B) from diseased (closed bars) and control (open bars) elderly and young groups were isolated and, immediately, their viability was analyzed by positivity to annexin V-FITC and propidium iodide. The cells were analyzed by fluorescence microscopy and the percent of apoptotic cells calculated by the proportion of positive neutrophil (green and/or red cells) in relation to total neutrophils. Results are expressed as the mean ± SEM from each group, elderly (n = 14) and young (n = 14) patients or aged (n = 14) and young (n = 14) control subjects tested individually. The results were evaluated by one-way ANOVA followed by Tukey's test and the value of P was considered significant when < 0.01. Equal symbols indicate significant difference between the groups.