Virus-specific CD8 T cells are generated in aged mice following de novo γHV68 infection. MHC class I tetramer staining and flow cytometric analyses were performed to determine the absolute numbers of ORF6/p56- and ORF61/p79-specific CD8 T cells present in the lungs (A, B) and spleens (C, D) of C57BL/6 mice infected when young (3 months old, closed symbols) or aged (20 months old, open symbols) at the indicated days following de novo γHV68 infection. Symbols represent data obtained from individual mice and bars indicate the medians. Significance was assessed using the Mann-Whitney (two-tailed, 95% confidence). Ns = not significant, * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001.