Table 2 Fluorescence intensity analysis after PNGase F treatment
From: Altered glycosylation profile of purified plasma ACT from Alzheimer’s disease
PANEL A | CTR 1 | AD 1 | ||||||
|---|---|---|---|---|---|---|---|---|
Band | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h |
1 | 12271 (41.2%) | 11658 (38.6%) | 8608 (57%) | 978 (5.5%) | 20528 (40.5%) | 16066 (37.4%) | 10821 (30.5%) | 10480 (25.3%) |
2 | 5297 (17.8%) | 5272 (17.4%) | 4313 (28%) | 9264 (52.6%) | 10135 (20%) | 5651 (13.1%) | 12959 (36.6%) | 15418 (37.1%) |
3 | 12229 (41%) | 13257 (44%) | 2240 (15%) | 4689 (26.6%) | 19972 (39.5%) | 21315 (49.5%) | 11668 (32.9%) | 15594 (37.6%) |
4 | 2702 (15.3%) | |||||||
PANEL B | CTR 2 | AD 2 | ||||||
Band | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h |
1 | 18537 (46.7%) | 12721 (44.9%) | 6925 (25.1%) | 5234 (15.7%) | 10371 (44.4%) | 10937 (37.2%) | 6649 (37.1%) | 5579 (36.7%) |
2 | 6293 (15.9%) | 2825 (10%) | 11136 (40.4%) | 14381 (43.3%) | 2916 (12.5%) | 4048 (13.8%) | 5527 (30.8%) | 3913 (25.8%) |
3 | 14817 (37.4%) | 12804 (45.1%) | 6094 (22.1%) | 10005 (30.1%) | 10083 (43.1%) | 14385 (49%) | 5759 (32.1%) | 5706 (37.5%) |
4 | 3428 (12.4%) | 3632 (10.9%) | ||||||