Figure 2
mRNA levels of IFNGR1, ADORA2A and IL6 over time in CMV-infected fibroblasts. Human lung fibroblasts were in vitro infected with CMV. Cells were harvested at the indicated time points, total RNA was isolated and gene expression of (A) IFNγ receptor 1 (IFNGR1), (B) adenosine A2A receptor (ADORA2A) and (C) Interleukin-6 (IL6) was analyzed using a RT2 Profiler PCR Array. Gene expression of CMV-infected fibroblasts was calculated in relation to gene expression of mock-infected fibroblasts. IFNGR1 and ADORA2A were maximally upregulated 24 hours post infection. While IFNGR1 expression returned to basal levels after 48 hours, ADORA2A was relatively stable expressed over time. In contrast IL6 showed a continuous upregulation of gene expression with a maximum at 72 hours following CMV-infection. The arithmetic mean ± S.E.M. were calculated for each examined group and time point (three independent experiments).