Figure 6

Involvement of IGF-1R/Fyn in the traumatic stress mediated neuroimmune modulation. 2-month and 1-year mice were subjected to surgical trauma, some of these mice were injected icv with IGF-1. Thus, 5 groups of mice were created: Con, T1, T1 + IGF-1, T3, T3 + IGF-1 (n = 5 for each group). Homogenates of spleen were prepared, and lymphocyte proliferation (A) and NK cell activity (B) were assayed by [³ H] incorporation. Fyn (+/−) and Fyn (−/−) mice were subjected to surgical trauma, some of these mice were injected icv with IGF-1. Thus, 5 groups of mice were created: Con, T1, T1 + IGF-1, T3, T3 + IGF-1 (n = 5 for each group). Homogenates of spleen were prepared, and lymphocyte proliferation (C) and NK cell activity (D) were assayed by [³ H] incorporation. Data are presented as percentage of control. Values represent mean ± SD for 3 independent experiments. *p < 0.05 vs Con, #p < 0.05 vs traumatized. Con: control; T1 and 3: 1 and 3 days after trauma.