Figure 1
From: Defective pro-IL-1β responses in macrophages from aged mice
Macrophages from aged mice produce less functional IL-1β but have similar levels of active Caspase-1. BMM were primed with LPS (200 ng/ml) for 3 hours and stimulated with ATP (5 mM) for one hour. IL-1β levels in the supernatant of stimulated BMM determined by ELISA (A). Data shown are the mean values from triplicate wells +/− SEM. Representative data of at least 3 experiments each performed in triplicate. Immunoblot analysis of the fully processed 17 kDa fragment of IL-1β in supernatants of BMM from young and aged mice stimulated with LPS + ATP (B). Immunoblot of active Caspase-1 (p20) subunit and pro Caspase 1 intermediate (p35) in the supernatants from stimulated young and aged BMM (C).