Figure 3
The percentage of marginal zone B cells was decreased in Ziz2 KO mice. (A) Splenocytes were analyzed by flow cytometry for T1 and MZ B cells in CD23− cells. T1: IgM+ CD21−. MZ: IgM+ CD21+. (B) The percentage of MZ B cells was decreased in Ziz2 KO mice. (C) Splenocytes were analyzed by flow cytometry for T2 (Transition 2) and mature (M) B cells in CD23+ cells. T2: IgMhigh CD21+. M: IgMlow/middle CD21+. (D) The percentage of “M” was not altered in KO mice. (E) Cells from the peritoneal cavity were analyzed by flow cytometry for B1a B cells in B220+ cells. B1a: B220middle CD5middle. (F) The percentage of B1a cells was not altered among the groups. 2KO: Ziz2 KO. 3KO: Ziz3 KO. *: P < 0.05 Three mice (8 weeks old, female) per group from three independent experiments (one mouse per group per experiment) were used.