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Figure 2 | Immunity & Ageing

Figure 2

From: Blood–brain barrier dysfunction developed during normal aging is associated with inflammation and loss of tight junctions but not with leukocyte recruitment

Figure 2

Cerebrovascular tight junction proteins and leukocyte recruitment. BBB tight junction assembly was assessed by measuring endothelial cell expressions of occludin-1 and ZO-1 with flow cytometry in the brains of 3 months old young mice and 24 months old aged mice. Example plots are in (A) and the fluorescent intensity of protein of interest is expressed as per endothelial cell (B). Asterisks indicate statistical significance assessed with two-tailed t-test (p < 0.05, n = 6). (C) Endothelial expression of adhesion proteins, ICAM-1 and VCAM-1, was measured with flow cytometry. (D) The infiltration of leukocytes across the BBB was assessed with immunomicroscopy staining of CD45 immunoreactivity within the perivascular region of entire cortex and hippocampal formation. Representative images are shown. Scale bar indicates 20 μm.

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Immunity & Ageing

ISSN: 1742-4933

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