Fig. 3

CASAC enhances CD8+ T cell responses to a tumour-associated epitope in aged mice. Aged C57BL/6 mice were vaccinated four times with the SVL peptide from TRP-2 in combination with either CFA/IFA (circles) or CASAC (triangles). a The percentage of H-2K^b/SVL-pentamer + CD8+ T cells was assessed after staining with H-2K^b/SVL-pentamer. b % Lysis of target cells was assessed (as explained in the legend to Fig. 2c) using CFSE^low–SVL-loaded and CFSE^high-SIL-loaded splenocytes as antigen-specific and control targets, respectively. c Production and intracellular accumulation of IFN-γ in CD8⁺ T cells in response to the vaccinated peptide was assessed using flow cytometry, after in vitro SVL peptide stimulation of splenocytes for 5 h. Each symbol represents an individual mouse and the median is indicated by a horizontal line. Data is pooled from 2 independent experiments. The Mann–Whitney U test was used to compare distributions