Fig. 3
From: A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats
Effects of a high fat diet (HFD) vs. standard diet (SD) on percentage of splenic total and apoptotic B cells over time. a Density plots depicting gating strategy for B220+ cells in the spleen (left plot) and analysis of early (Annexin V+/7-AAD-) and late (Annexin V+/7-AAD+) apoptotic B cells in the pleen (right plot). b, c Circles represent mean values at different age groups [grey circle: baseline value, closed circles: HFD, open circles: SD]. b percentage of splenic B220+ B cells. c percentage of early apoptotic (left graph) or late apoptotic (right graph) B cells in the spleen. ANOVA was used to determine overall difference between dietary regimen and age groups. Error bars represent the S.E.M. Young: 2 month, old: 18–24 month