Fig. 5
Aging increases the number of all CD11b + nonlymphoid cells, and CD45hi CD11b + cells, recovered on the 16th d.p.i. from AO rat spinal cord. (Panel a) Flow cytometry density plots indicate the expression of CD45 on CD11b + cells from spinal cord of (left) young and (right) aged rats on the 16th d.p.i. CD11b + cells were gated as shown in the flow cytometry histograms. Numbers in the flow cytometry histograms represent the percentage of CD11b + cells. R1 = CD11b + CD45hi cells; R2 = CD11b + CD45int cells. Numbers in the table represent the percentage of cells in the indicated region (R). Bar graphs indicate the number of (upper) CD11b + and (lower left) CD11b + CD45int and (lower right) CD11b + CD45hi spinal cord mononuclear cells retrieved from young and aged rats. (Panel b) Bar graphs indicate the fold change in mRNA expression for (a) CCL2 in spinal cord tissue and (b) SOCS1 and (c) TNFα in spinal cord mononuclear cells from aged relative to young rats on the 16th d.p.i. as determined by RT-qPCR. Data are normalized to β-actin (ACTB). All results are presented as means ± SEM (n = 9/group). Data are representative of one of two experiments with similar results. ***p < 0.001