Fig. 7
Aging increases the number of CD134 + CD4+ T lymphocytes in draining lymph nodes of AO rats immunized for EAE. (Panel a) Flow cytometry dot plots show CD4 vs TCRαβ staining of draining lymph node mononuclear cells from (left) young and (right) aged rats (upper dot plots) injected with CFA and Bordetella pertussis (Adjuvant) or (lower dot plots) immunized for EAE (Immunized) on the 7th d.p.i. Bar graph shows the number of CD4+ TCRαβ + cells in draining lymph nodes from young and aged rats injected with adjuvant or immunized for EAE. (Panel b) Flow cytometry dot plots show CD134 vs CD4 staining of CD4+ TCRαβ + lymphocytes retrieved from draining lymph nodes of (left) young and (right) aged rats (upper dot plots) injected with CFA and Bordetella pertussis (Adjuvant) or (lower dot plots) immunized for EAE on the 7th d.p.i. Bar graph shows the number of CD134 + CD4+ TCRαβ + cells in draining lymph nodes from young and aged rats injected with adjuvant or immunized for EAE. Numbers in the flow cytometry dot plots represent the percentage of cells in the indicated region. All results are presented as means ± SEM (n = 9/group). Data are representative of one of two experiments with similar results. **p < 0.01; ***p < 0.001