Table 1 Forward and reverse primer sequences used on the quantitative PCR and RT-PCR assays
From: Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
Target | Sequence forward (5'-3') | Sequence reverse (5'-3') | Amplicon size (pb) | Reference |
|---|---|---|---|---|
FOXN1 (NM_003593.2) | TCCCTCACTCACTGACTTCG (1628–1647) | GTGGCATCGAAGATGATGTC (1746–1727) | 119 | [72] |
DLL1 (NM_005618.3) | TGCAACCAGGACCTGAACTA (1323–1342) | CTCCGTTCTTACAAGGGCTG (1491–1472) | 163 | * |
DLL4 (NM_019074.3) | CAGAGTGTCGGATATCAGCG (2288–2307) | CTCCTGCCTTATACCTCCGT (2402–2383) | 115 | * |
WNT-4 (NM_030761.4) | CAGCAGAGCCCTCATGAACC (647–666) | GCCAGCACGTCTTTACCTCACA (768–747) | 122 | [73] |
HPRT-1 (NM_000194.2) | CCTGGCGTCGTGATTAGTG (183–201) | TCGAGCAAGACGTTCAGTCC (320–301) | 138 | * |
TFRC (NM_001128148.1) | CTAGTGTTCTTCTGTGTGGCAGTT (115–138) | ACAATGGTTCTCCACCAAACAAG (197–175) | 83 | * |
RPL13A (NM_001270491.1) | CGGACCGTGCGAGGTATGCT (244–263) | AGCAGGAACCACCATCCGCT (366–347) | 123 | * |