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Fig. 1 | Immunity & Ageing

Fig. 1

From: Efforts of the human immune system to maintain the peripheral CD8+ T cell compartment after childhood thymectomy

Fig. 1

Analysis of CD127+, CD31+ and CD103+ CD8+ T cell proportions. Representative examples of flow cytometric analysis of CD8+ T cells of one YATEC patient, one YHC and one OHC are shown. Gating strategies were as follows: First, CD8+ T cells were analyzed within the CD3+ lymphocyte gate. Percentages of CD45RA+ and CD45RA- were analyzed in CD8+ within the CD3+ T cell gate (a). CD127+ was determined within CD45RA+ (b) and CD45RA- CD8+ T cells (c) together with CD31, percentages indicating positive events in the CD45RA+ or CD45RA- CD8+ T cell gate, respectively. Due to low percentages of circulating CD103 + CD8+, these cells were determined in the total lymphocyte gate (d). Percentages of Ki67+ were determined together with CD127+ in the CD45RA+ (e) and CD45RA- CD8+ gate (f), respectively

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Immunity & Ageing

ISSN: 1742-4933

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