Fig. 4

AR silencing prevents DHEA-induced RACK1 expression. THP-1 cells were transfected with transfection reagent alone (no oligo) or with siRNA (60 nmol) targeted to AR (siAR) or with control siRNA (scr). After 48 h, extracts were prepared for Western blot analysis of AR, RACK1 and β-actin (loading control) immunoreactivity to estimate AR knockdown efficiency (inset). After silencing, cells were incubated with DHEA (10 nM) or DMSO as vehicle control for 16 h to assess mRNA expression (a) and 24 h to assess protein expression (b). Results are expressed as mean ± SD of three independent experiments, with *p < 0.05 and **p < 0.01 vs control cells, and §p < 0.05 vs no oligo or siSCR treated cells