Fig. 1

Gating strategy for flow cytometric analyses of NK cells. a. Lymphocyte gating - lymphocytes were defined as FSC^low/SSC^low cells (G1). b. NK cell gating – NK cells were defined as CD3 negative and CD56 positive population (G2). c. NK cell dim/bright gating – NK cells were defined as CD3 negative and CD56 dim positive (G3) or CD56 bright positive population (G4) within NK cell population. d. NK cells expressing SIRT1 were identified in upper right quadrant (Q2). e. NK cells expressing SOD2 were identified in upper right quadrant (Q2). f. NK cells expressing intracellular HSP70 were identified in upper right quadrant (Q2). g. Isotype control for SIRT1 positive cells (Q3). h. Isotype control for SOD2 positive cells (Q3). i. Isotype control for HSP70 positive cells (Q3)