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Table 2 Overview of staining panels and reagents for flowcytometry

From: Changes in peripheral immune cell numbers and functions in octogenarian walkers – an acute exercise study

Panel Mab reagent Clone Provider
T cell differentiation CD3-Efluor 605 okt-03 Ebioscience, San Diego, CA, USA
  CD8-APC-H7 RPA-T8 Ebioscience, San Diego, CA, USA
  CD45RO-FITC UCHL-1 BD bioscience, San Jose, CA, USA
  CCR7-PE-Cy7 3D12 BD bioscience, San Jose, CA, USA
  CD31-AF647 WM-59 BD bioscience, San Jose, CA, USA
  CD28-AF700 28.2 Biolegend, San Diego, CA, USA
  PD1-PE EH12.2H7 Biolegend, San Diego, CA, USA
  CD4-PcP SK3 BD bioscience, San Jose, CA, USA
  CTLA-4-BV421 BNI3 BD bioscience, San Jose, CA, USA
Treg/Proliferation CD8-PE-Cy7 RPA-T8 BD bioscience, San Jose, CA, USA
  CD25-APC BC96 Ebioscience, San Diego, CA, USA
  CD45RA-Efluor605 HI100 Ebioscience, San Diego, CA, USA
  CD19-FITC HD37 Dako, Santa Clara, CA, USA
  CD4-APC-H7 RPA-T4 BD bioscience, San Jose, CA, USA
  FOXP3-PE PCH101 BD bioscience, San Jose, CA, USA
  Ki-67-PcP-Cy5.5 B56 BD bioscience, San Jose, CA, USA
NK cell CD16-FITC 3G8 Beckman Coulter, Brea, CA, USA
Inhibitory receptors CD56-ECD N901 Beckman Coulter, Brea, CA, USA
  CD3-APC-AF750 UCHT1 Beckman Coulter, Brea, CA, USA
  CD45-KO J.33 Beckman Coulter, Brea, CA, USA
  CD159c (NKG2C)-PE 134591 R&D Systems, Minneapolis, MN, USA
  CD158b (KIR2DL2/3)-PE-Cy7 GL183 Beckman Coulter, Brea, CA, USA
  CD158e1 (KIR3DL1)-APC Z27.3.7 Beckman Coulter, Brea, CA, USA
  CD158a (KIR2DL1)-APC-AF700 EB6B Beckman Coulter, Brea, CA, USA
  CD159a (NKG2A)-PB Z199 Beckman Coulter, Brea, CA, USA
NK cell CD16-FITC 3G8 Beckman Coulter, Brea, CA, USA
Activating receptors CD56-ECD N901 Beckman Coulter, Brea, CA, USA
  CD3-APC-AF750 UCHT1 Beckman Coulter, Brea, CA, USA
  CD45-KO J.33 Beckman Coulter, Brea, CA, USA
  CD336 (NKp44)-PE Z231 Beckman Coulter, Brea, CA, USA
  CD337 (NKp30)-PE-Cy5.5 Z25 Beckman Coulter, Brea, CA, USA
  CD335 (NKp46)-PE-Cy7 BAB281 Beckman Coulter, Brea, CA, USA
  CD314 (NKG2D)-APC ON72 Beckman Coulter, Brea, CA, USA
  CD244 (2B4) -APC-AF700 C1.7.1 Beckman Coulter, Brea, CA, USA
  CD161-PB 191B8 Beckman Coulter, Brea, CA, USA