Changes in peripheral immune cell numbers and functions in octogenarian walkers – an acute exercise study

van der Geest, Kornelis S. M.; Wang, Qi; Eijsvogels, Thijs M. H.; Koenen, Hans J. P.; Joosten, Irma; Brouwer, Elisabeth; Hopman, Maria T. E.; Jacobs, Joannes F. M.; Boots, Annemieke M. H.

doi:10.1186/s12979-017-0087-2

Immunity & Ageing

Table 2 Overview of staining panels and reagents for flowcytometry

From: Changes in peripheral immune cell numbers and functions in octogenarian walkers – an acute exercise study

Panel	Mab reagent	Clone	Provider
T cell differentiation	CD3-Efluor 605	okt-03	Ebioscience, San Diego, CA, USA
	CD8-APC-H7	RPA-T8	Ebioscience, San Diego, CA, USA
	CD45RO-FITC	UCHL-1	BD bioscience, San Jose, CA, USA
	CCR7-PE-Cy7	3D12	BD bioscience, San Jose, CA, USA
	CD31-AF647	WM-59	BD bioscience, San Jose, CA, USA
	CD28-AF700	28.2	Biolegend, San Diego, CA, USA
	PD1-PE	EH12.2H7	Biolegend, San Diego, CA, USA
	CD4-PcP	SK3	BD bioscience, San Jose, CA, USA
	CTLA-4-BV421	BNI3	BD bioscience, San Jose, CA, USA
Treg/Proliferation	CD8-PE-Cy7	RPA-T8	BD bioscience, San Jose, CA, USA
	CD25-APC	BC96	Ebioscience, San Diego, CA, USA
	CD45RA-Efluor605	HI100	Ebioscience, San Diego, CA, USA
	CD19-FITC	HD37	Dako, Santa Clara, CA, USA
	CD4-APC-H7	RPA-T4	BD bioscience, San Jose, CA, USA
	FOXP3-PE	PCH101	BD bioscience, San Jose, CA, USA
	Ki-67-PcP-Cy5.5	B56	BD bioscience, San Jose, CA, USA
NK cell	CD16-FITC	3G8	Beckman Coulter, Brea, CA, USA
Inhibitory receptors	CD56-ECD	N901	Beckman Coulter, Brea, CA, USA
	CD3-APC-AF750	UCHT1	Beckman Coulter, Brea, CA, USA
	CD45-KO	J.33	Beckman Coulter, Brea, CA, USA
	CD159c (NKG2C)-PE	134591	R&D Systems, Minneapolis, MN, USA
	CD158b (KIR2DL2/3)-PE-Cy7	GL183	Beckman Coulter, Brea, CA, USA
	CD158e1 (KIR3DL1)-APC	Z27.3.7	Beckman Coulter, Brea, CA, USA
	CD158a (KIR2DL1)-APC-AF700	EB6B	Beckman Coulter, Brea, CA, USA
	CD159a (NKG2A)-PB	Z199	Beckman Coulter, Brea, CA, USA
NK cell	CD16-FITC	3G8	Beckman Coulter, Brea, CA, USA
Activating receptors	CD56-ECD	N901	Beckman Coulter, Brea, CA, USA
	CD3-APC-AF750	UCHT1	Beckman Coulter, Brea, CA, USA
	CD45-KO	J.33	Beckman Coulter, Brea, CA, USA
	CD336 (NKp44)-PE	Z231	Beckman Coulter, Brea, CA, USA
	CD337 (NKp30)-PE-Cy5.5	Z25	Beckman Coulter, Brea, CA, USA
	CD335 (NKp46)-PE-Cy7	BAB281	Beckman Coulter, Brea, CA, USA
	CD314 (NKG2D)-APC	ON72	Beckman Coulter, Brea, CA, USA
	CD244 (2B4) -APC-AF700	C1.7.1	Beckman Coulter, Brea, CA, USA
	CD161-PB	191B8	Beckman Coulter, Brea, CA, USA

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ISSN: 1742-4933

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