Transcriptomic evidence of a para-inflammatory state in the middle aged lumbar spinal cord

Immunity & Ageing

Table 3 Canonical pathways populated by the age-related gene set

Pathway	Target Genes	Pathway Genes	Ratio	z-score	B-H p value	Target Gene ID’s
Complement	9	37	0.243	1.414	1.11E-06	C3, C1QA, C1QB, C1QC, C1S, CFD, CFH, ITGB2, SERPING1
Cholesterol Biosynthesis	7	28	0.250		2.63E-05	CYP51A1, HMGCR, HMGCS1, HSD17B7, IDI1, LSS, SC5D
iCOS-iCOSL signaling T helper cell	10	108	0.093		6.64E-04	CAMK2B, CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, PDPK1, PLEKHA4, PTPRC
Cholesterol Biosynthesis I	4	13	0.308		1.84E-03	CYP51A1, HSD17B7, LSS, SC5D
Cholesterol Biosynthesis II (via 24,25-dihydrolanosterol)	4	13	0.308		1.84E-03	CYP51A1, HSD17B7, LSS, SC5D
Cholesterol Biosynthesis II (via desmosterol)	4	13	0.308		1.84E-03	CYP51A1, HSD17B7, LSS, SC5D
Ca-induced T cell Apoptosis	7	64	0.109		2.40E-03	CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, PRKCE
OX40-signaling	8	89	0.090		2.40E-03	CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, RT1-EC2, TNFSF4
NFAT regulation of immune response	11	171	0.064	1.89	2.40E-03	BLNK, CD3G, FCER1G, FCGR2B, FCGR3A/FCGR3B, GNAS, GSK3B, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5
B Cell development	5	33	0.152		4.35E-03	HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, PTPRC
Nur77 signaling in T cells	6	57	0.105		6.70E-03	CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5
Cd28 signaling in T Helper Cells	8	118	0.068		1.15E-02	CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, PDPK1, PTPRC
PKC-τ signaling in T cells	8	118	0.068	1.414	1.15E-02	CAMK2B, CD3G, FCER1G, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB5, MAP3K8

The differentially expressed annotated genes (target gene set) were placed in the contexts of canonical biological pathways using the IPA software and its curated database. Ratio = target genes/pathway genes. P values were calculated using a modified Fisher test that corrects for multiple hypothesis testing. Significant Z-score indicate overall pathway modulation (+) for up (-) for down

ISSN: 1742-4933