Fig. 4
Effect of TNF-α on the phosphorylation of JNK, IKKβ, IκB in TN and TCM CD8+ T cells. Purified TN and TCM CD8+ T cells from young and aged subjects were stimulated with TNF-α for 10 min and then analyzed for expression of phospho JNK, IKKβ, IκB, using specific antibodies. β-actin was used as a loading control. [a] Western blot from one such experiment, [b] densitometry data, and [c] shows densitometry data from 5 each young and aged subjects. A significant decrease in pJNK, pIKKβ, and pIκB (P < 0.05, P < 0.01) was observed in aged subjects as compared to young subjects