Fig. 5

Concentration of protein carbonyl groups and 8-isoprostanes in cell extracts prepared from non-stimulated and stimulated NK cells of the young, the old aged under 85 and the oldest (aged over 85). Data are presented as means ± SEM. The same symbols over bars ^X, ^<, ^T, ^o, ^Δ, ^V, ⁺denote statistically significant differences between similarly treated NK cells of different age groups (i.e. young vs old; young vs oldest or old vs oldest). Horizontal lines above paired bars denote statistically significant differences between treated and untreated NK cells of the same age group (i.e. non-stimulated vs stimulated with IL-2, LPS or PMA with ionomycin). a The concentration of protein carbonyl groups in cell extracts of NK cells expressed as nmol/mg protein: ^Δ, ⁺, ^op ≤ 0.01; ^x, ^T, ^<, ^Vp < 0.05. b. The concentration of 8-isoprostanes in cell extracts of NK cells expressed as pg/mL: ^<, ^Δ, ^xp ≤ 0.001; ^Yp < 0.05