Fig. 5

VM cells from aged mice express Granzyme B following response to influenza infection. a Pooled CD8 VM cells (CD44^High/CD62L^High/CD49d^Low) from aged C57BL/6 donors and pooled CD8 naïve cells (CD44^Low/CD62L^High) from young C57BL/6 donors were FACS-sorted and adoptively transferred into individual T cell deficient TCR βδ−/− mice, infected with influenza, and assessed for Granzyme B expression at days 6 and 9 post infection. b Representative histograms gated on the donor CD8 T cell population show Granzyme B expression in the lung at the indicated time points after infection. c Summary graphs show plots of of Granzyme B expression (gMFI) in lung donor CD8 T cell populations from individual recipient mice at the indicated times after infection. Data from two independent experiments, pooled for statistical analysis, are represented in separate shades. Bars represent the mean ± SD. Data were analyzed by unpaired, two-tailed T test (p < 0.05)