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Table 2 Repeated measurements of the cytokine concentrations, overall and by sex, with estimated ICCs

From: Cytokines for evaluation of chronic inflammatory status in ageing research: reliability and phenotypic characterisation

Cytokines (pg/ml)

First measurement

Second measurement

P difference*

ICC (95% CI)

N

Median (IQR)

N

Median (IQR)

Interleukin-1beta

ALL

79

3.12 (0.35–6.50)

82

3.00 (0.39–5.45)

0.89

0.25 (0.02, 0.44)

Men

31

2.71 (0.35–4.42)

30

3.27 (0.23–6.25)

0.72

0.28 (−0.11, 0.57)

Women

48

3.47 (0.44–7.18)

52

2.97 (0.53–5.18)

0.92

0.23 (−0.07, 0.47)

P difference**

 

0.32

 

0.77

  

Interleukin-2

ALL

143

2.13 (0.21–6.47)

131

1.77 (0.19–5.23)

0.72

0.32 (0.16, 0.46)

Men

59

2.32 (0.21–6.23)

56

2.15 (0.21–4.66)

0.93

0.26 (0.00, 0.48)

Women

84

1.67 (0.20–6.52)

75

0.56 (0.19–6.37)

0.62

0.36 (0.15, 0.54)

P difference

 

0.82

 

0.83

  

Interleukin-4

ALL

143

2.66 (1.53–5.75)

152

2.75 (1.57–5.98)

0.94

−0.18 (−0.34, −0.02)

Men

56

2.69 (1.52–6.02)

60

2.70 (1.47–5.49)

0.78

−0.23 (−0.47, 0.02)

Women

87

2.63 (1.53–5.61)

92

2.90 (1.65–6.15)

0.64

−0.15 (−0.35, 0.06)

P difference

 

0.92

 

0.78

  

Interleukin-6

ALL

207

0.52 (0.41–0.79)

207

0.52 (0.40–0.71)

0.06

0.60 (0.51, 0.68)

Men

83

0.59 (0.44–0.82)

83

0.60 (0.44–0.81)

0.55

0.51 (0.34, 0.66)

Women

124

0.50 (0.37–0.76)

124

0.47 (0.38–0.68)

0.04

0.63 (0.51, 0.72)

P difference

 

0.02

 

0.001

  

Interleukin-8

ALL

207

3.91 (3.10–4.88)

207

3.89 (2.99–5.02)

0.32

0.64 (0.55, 0.71)

Men

83

3.96 (3.19–4.96)

83

4.19 (3.20–5.62)

0.07

0.62 (0.47, 0.74)

Women

124

3.67 (3.03–4.73)

124

3.62 (2.81–4.74)

0.81

0.63 (0.51, 0.73)

P difference

 

0.14

 

0.004

  

Interleukin-10

ALL

207

0.25 (0.18–0.34)

207

0.25 (0.18–0.34)

0.76

0.58 (0.48, 0.66)

Men

83

0.28 (0.19–0.36)

83

0.26 (0.19–0.35)

0.03

0.38 (0.18, 0.55)

Women

124

0.24 (0.17–0.31)

124

0.24 (0.18–0.33)

0.13

0.72 (0.63, 0.80)

P difference

 

0.02

 

0.35

  

Interleukin-12p70

ALL

194

0.30 (0.15–6.01)

203

0.31 (0.16–6.01)

0.98

0.35 (0.22, 0.47)

Men

78

1.90 (0.13–6.69)

81

1.06 (0.14–6.00)

0.29

0.27 (0.05, 0.46)

Women

116

0.22 (0.15–5.32)

122

0.27 (0.16–6.20)

0.30

0.40 (0.24, 0.54)

P difference

 

0.31

 

0.87

  

Interleukin-13

ALL

167

0.67 (0.43–0.92)

168

0.77 (0.52–1.04)

0.01

0.43 (0.30, 0.55)

Men

69

0.55 (0.41–0.76)

67

0.63 (0.44–0.97)

0.12

0.43 (0.21, 0.60)

Women

98

0.75 (0.49–0.20)

101

0.83 (0.61–1.08)

0.06

0.39 (0.21, 0.54)

P difference

 

0.01

 

0.01

  

Interferon-gamma

ALL

207

3.06 (2.12–4.61)

207

3.06 (2.18–4.21)

0.43

0.40 (0.28, 0.51)

Men

83

2.81 (2.09–4.29)

83

2.82 (2.06–3.87)

0.66

0.28 (0.07, 0.46)

Women

124

3.37 (2.16–4.86)

124

3.26 (2.22–4.44)

0.46

0.48 (0.33, 0.61)

P difference

 

0.28

 

0.14

  

Tumor Necrosis Factor alpha

ALL

207

1.94 (1.56–2.39)

207

1.96 (1.57–2.40)

0.07

0.70 (0.63, 0.77)

Men

83

2.04 (1.64–2.40)

83

2.14 (1.60–2.50)

0.44

0.77 (0.66, 0.84)

Women

124

1.87 (1.51–2.36)

124

1.87 (1.56–2.33)

0.10

0.65 (0.53, 0.74)

P difference

 

0.07

 

0.08

  
  1. *P value for difference based on Wilcoxon signed rank test between first and second measurements. **P value for difference based on Wilcoxon rank sum test between men and women (Kruskal Wallis) Abbreviations: ICC Intraclass correlation coefficient, IQR Interquartile range, CI Confidence interval
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Immunity & Ageing

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