Fig. 3

Kinetics of proliferative responses. Panel a shows the percentage of individuals in the Junior and Senior groups that showed proliferative responses at various time points upon stimulation with HBsAg at various time points. & indicate time points with ≥10% differences between Juniors and Seniors. Panel b shows the net proliferative responses (cpm) in Juniors and Seniors at 60, 210 and 360 days of the study (box and whisker plots). A WMW test was used to compare 2 groups (Seniors vs. Juniors). Significant differences (p < 0.05; *) between groups are indicated. Panels below show percent of vaccine recipients exhibiting proliferative responses by c route of vaccination (IM and SC), e sex (females and males), and g age subsets (65–74 and ≥ 75 years). + indicate time points with ≥10% differences between Senior subsets. Vaccination days are indicated with the Red Triangles. Panels d, f and h display the net proliferative responses (cpm) (days 60, 210 and 360) for Seniors split in subsets depending on route of vaccination, sex and age. Juniors and Seniors subsets at defined time points were compared using the Kruskal-Wallis test (#; p < 0.05) followed by the Dunn multiple comparisons procedure. Box and whisker plots display Min and Max values as well as the median. IM: Intramuscular SC: Subcutaneous. F: Females; M: males