Fig. 4

Overexpression of ARID3a in aged donor HSCs increases B lineage cell numbers. The aged donor HSCs from Fig. 3 were untreated (media, circles), or transfected with lentivirus vector controls (control, squares) or lentivirus containing full length ARID3a (ARID3a, triangles) for 24 h, plated as triplicate cultures and analyzed by flow cytometry. a Relative transfection efficiencies indicated by co-expression of RFP at the end of the cultures are shown. b Total numbers and frequencies of CD33⁺ myeloid cells (c), CD19⁺ B lineage cells (d), and CD56⁺ NK cells (e) are shown. Total frequencies of CD34 expressing cells (f) and percentages of lineage-directed cells co-expressing CD34 (g) are shown. Means and standard error bars are shown. Statistical significance was determined by Mann Whittney U test; **, p < 0.0093, ***, p < 0.0003