Fig. 2
From: Reduced immune-regulatory molecule expression on human colonic memory CD4 T cells in older adults
PB CD45RA- CD4 T cells have limited age-associated alterations in immune phenotype. PBMC were isolated from blood samples obtained from younger (Y; ≤45 yrs) and older (O; ≥65 yrs) persons (N = 9/age group unless otherwise stated) and multi-color flow cytometry used to evaluate baseline expression of a CTLA-4, PD-1, Ki67, Bcl-2 or b CD25, CD38 and HLA-DR, or CD57 (Y N = 8). Values are shown as the percentage of LP CD4 T cells expressing each marker or as total expression levels on LP CD4 T cells (Geometric Mean Fluorescence Intensity; GMFI). Isotype control (Bcl-2, CD38, Ki67, CTLA-4), Fluorescence minus one (FMO; CD25) or FM4 (HLA-DR, CD57, PD-1) values have been removed (net). Bar graphs represent mean ± SEM with individual samples shown as open triangles. Statistical analysis: unpaired t-tests