Fig. 2

Total B cells and ABCs from old mice express higher levels of RNA for pro-inflammatory markers, and markers of autoimmune antibody production, as compared to those from young mice. a. B cells were isolated by magnetic sorting using CD19 microbeads from the spleens of the same pairs of young and old mice shown in Fig. 1. B cells were left unstimulated, the mRNA was extracted and qPCRperformed to evaluate RNA expression of SASP markers. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). b. RNA expression of the same SASP markers in A in unstimulated FO and ABCs sorted from the spleens of young and old mice. c. RNA expression of Prdm1, Tbx21, Igtax, CD86, Kifc3, Syntaxin3 in unstimulated FO and ABCs sorted from the same mice in (b). Mean comparisons between groups were performed by two-way ANOVA. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001