Fig. 2

Decreased early immune mediator production and less inflammation in aged baboon skin challenged with TST. Levels of immune proteins in (a) ST 3-day biopsies and (b) LT 3-day biopsies from adult (A) and aged (O) vaccinated baboons were detected by Luminex assay. Shown for each analyte: protein concentration normalized per μg of protein in skin tissue homogenates (left) and the fold change of TST protein levels vs saline (right). One-way ANOVA post-Tukey analyses Adult TST vs Aged TST, *p < 0.05, **p < 0.01. H&E stained skin tissue from adult and aged baboons was evaluated for inflammation, cell infiltration, and skin structural changes in ST 3-day biopsies (c) and LT 3-day biopsies (d). Representative images are shown of adult (left) and aged (right) skin tissue in response to NaCl (top) and TST (bottom) for ST 3-day biopsies (c) and LT 3-day biopsies (d). Percent affected inflammation is quantified in ST 3-day biopsies (e) and LT 3-day biopsies (f)