Fig. 3

Increased aged PBMC migration in response to skin homogenates and functional PBMC responses to antigen. a Homogeneous migration of adult and aged PBMCs in response to skin tissue homogenates from TST and NaCl ST 3-day and 7-day biopsies and LT 3-day and 7-day biopsies. A is adult; O is aged. Each dot represents one animal. b Heterogeneous migration of adult and aged PBMCs in response to skin tissue homogenates from TST and NaCl ST 3-day and 7-day biopsies and LT 3-day and 7-day biopsies. Each dot represents a replicate, performed in triplicate, from adult or aged PBMCs by age group. Shown is fold change vs adult for homogeneous (A) or heterogenous (B) experimental setup. Student’s t test Adult TST vs Aged TST and Adult NaCl vs Aged NaCl, *p < 0.05, **p < 0.01, ***p < 0.001. c-e PBMCs from adult and aged vaccinated baboons from the time points indicated were stimulated with CFP for 5 days. Supernatants were collected and antigen-specific responses were detected by Luminex for production of (c,d) cytokines and (e) chemokines. Student’s t test Adult vs Aged PBMCs, *p < 0.05