Fig. 2

Effect of Young MDSPC Systemic Transplantation in the Zmpste24^−/− Progeroid Mouse Articular Cartilage Microenvironment. a Schematic illustrating the experimental design. b-d Quantitation of pro-inflammatory cytokines and SASP markers (Il1a, Il6, and Tnf) and anti-inflammatory cytokine (Il10), extracellular matrix (ECM) proteinases (Mmp3, Mmp13, and Adamts5), and ECM components (Col1a1, Col2a1, Acan, Vcan, and Bgn) relative mRNA levels, measured by qRT-PCR from the knee articular cartilage of Zmpste24^−/− mice intraperitoneally (IP) injected with MDSPCs (Z-IP, n = 7) or PBS (Z-PBS, n = 9). Expression values are relative to housekeeping genes, Gapdh and Hmgb1. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.0001, ^§p < 0.00001, ^ǂp < 0.000001, ns: not significant using two-tailed, unpaired Student’s t-test or Welch’s unequal variance t-test