Fig. 2

Moderate ethanol fails to induce ileal Cldn1 and Reg3 gene expression in aged mice. Quantitative RT-PCR of tight junction (A-C, n = 4) and AMP (D-E, n = 6–10) gene expression in intestinal epithelial cells (IECs) isolated from young and aged mice given vehicle or ethanol. 18 s was used as the endogenous control. Data are shown as mean fold change ± SEM relative to young vehicle group. *p < 0.05 from all other groups, #p < 0.05 from young vehicle and aged ethanol by two-way ANOVA with Tukey’s multiple comparisons test