Fig. 5

Effect of ACA, Rapa and 17aE2 treatments on liver eIF4E signaling in liver. A Representative western blots for phospho-eIF4E at Serine 209 (peIF4E) and total levels of eIF4E protein in liver samples from young untreated (Y), old untreated (O), old treated with ACA or Rapa (O, early and late) and 17aE2 (early and late) treatments separated by sex (males = M, females = F). B Bar graphs represent the mean ± SEM of peIF4E ratio and eIF4E protein levels in liver of 8 young and 8 old male mice plus, at least, 4 mice for each of the ACA and Rapa and 17aE2 male treated groups. All values have been normalized to young control females as described in the Methods section. C Bar graphs represent the mean ± SEM of peIF4E ratio and eIF4E protein levels in liver of 8 young and 8 old female mice plus, at least, 4 mice for each of the ACA and Rapa and 17aE2 female treated groups. The (*) indicates statistical significance (p < 0.05) in a t-test between the indicated pair of groups