Fig. 3

Age-associated accumulation of CD44^hi CD27^neg Vγ4 T cells in naïve mice. Vγ4 T cells in the MLN of naïve B6 mice of different ages were analyzed. A Representative flow plots for the indicated age groups are shown. Compounded frequency of CD44^hi CD27^neg Vγ4 T cells among total γδ T cells (B) and absolute numbers (C) of CD44^hi CD27^neg Vγ4 T cells are shown as mean ± SEM. All data sets in (B-E) are compiled from at least 3 independent experiments with a minimum of 3 mice/group, except 25-26-month-old B6 (1 experiment, 3 mice). D Linear regression between CD44^hi CD27^neg Vγ4 T cell numbers and age is shown. E Linear regression between CD44^hi CD27^neg Vγ4 T cell numbers and weight is shown. F Body weight for each age group is shown as mean ± SEM. G Linear regressions between CD44^hi CD27^neg Vγ4 T cell numbers and weight by indicated age groups are shown. H Frequency of CD127^hi cells is shown as mean ± SEM. Each symbol represents an individual mouse. All data sets in (F-H) are compiled from at least 3 independent experiments with a minimum of 3 mice/group, except 7-10-month-old B6 (1 experiment, 5 mice) and 25-26-month-old B6 (1 experiment, 3 mice)