Fig. 7
Infection of aged mice with Lm ΔactA increases numbers of cytokine-expressing CD8+ bTRM. Male C57BL/6JN mice aged 19 mo were infected with 2.1 × 107 CFU Lm ΔactA (●), or injected with PBS as uninfected controls (○). All mice received amoxicillin in their drinking water and brain leukocytes were analyzed by FACS. Figs. (A-C) show results of intracellular staining of gated CD8+ bTRM for IFNγ (A), TNF (B), and granzyme B (C). Symbols represent individual mice. Line indicates mean. Statistical significance calculated via Student’s t test