Fig. 8
miR-155 inhibitor suppresses myeloid cell accumulation in brains of aged mice infected with Lm ΔactA. 22–23 mo old male C57BL/6JN mice were infected with 2.1 × 107 CFU Lm ΔactA or injected with PBS as uninfected controls. All mice received amoxicillin in their drinking water beginning 3d p.i., and mice were given injections s.c. of miR-155 inhibitor (▼, n = 10), scramble (▲, n = 10), or PBS (○, n = 9) 2d, 4d, 6d, and 8d p.i. (A) Weight was measured daily and each symbol represents the mean % starting weight/cohort for that day. Statistical significance calculated via 2-way ANOVA. Uninfected v miR-155 inhibitor p < 0.0001; Uninfected v LNA scramble p < 0.0001; miR-155 inhibitor v LNA scramble ns. (B) Kaplan-Meier survival curves for each group. Brain leukocytes collected 29d – 38d p.i., were analyzed by FACS (C-I). Symbols represent individual mice, lines indicate group mean. P values shown were calculated with Student’s t test or Mann-Whitney U test