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Fig. 3 | Immunity & Ageing

Fig. 3

From: Poly-γ-glutamic acid/Alum adjuvanted pH1N1 vaccine-immunized aged mice exhibit a significant increase in vaccine efficacy with a decrease in age-associated CD8+ T cell proportion in splenocytes

Fig. 3

PGA/Alum significantly improves influenza pH1N1 vaccine antigen-specific cell-mediated and humoral immune responses in aged mice. Aged mice (n = 5–10 per group) were i.m. administered the pH1N1 split vaccine antigen alone or mixed with γ-PGA, alum, or PGA/Alum on days 0 and 14. The splenocytes and sera were collected 2 weeks after the last immunization. A The splenocytes were stimulated with 500 TCID50 UV-inactivated pH1N1 for 60 h, and IFN-γ+ SFUs were detected via ELISPOT assays. B Serum HI titers were measured against the pH1N1 using the sera and are shown as GMT. (C and D) ELISA was performed to determine sera Ab titers of vaccine antigen-specific IgG (C), IgG1, and IgG2b (D). Statistical significance was analyzed using one-way ANOVA/Bonferroni; *P < 0.05, **P < 0.01, and ***P < 0.001

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Immunity & Ageing

ISSN: 1742-4933

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