Fig. 5

Confirmation of WGBS data by BS-PCR and qPCR. A DNA methylation and gene expression profiles in the promoter region of stat1b gene. B DNA methylation and gene expression profiles in the promoter region of trap1 gene. C DNA methylation and gene expression profiles in the gene body region of gapdh gene. D DNA methylation and gene expression profiles in the gene body region of idha gene. Top panels: schematic diagram of promoter regions or gene body regions of four genes. The circles indicate the candidate CpG loci in the DMRs. Middle panels: Validation of CpG methylation by BS-PCR. Fifteen subclones from each group were selected for the BSP analysis. The solid circles represent the methylated CpG sites, and the open circles indicate the unmethylated CpG sites. Bottom panels: Comparison of gene expression levels between two groups by qPCR