Fig. 6
The antiviral effects of two metabolism-related genes. A Western blotting confirmation of the overexpression of two genes in transfected cells. Cells were transfected with the corresponding plasmids for 24 h and harvested for western blotting analysis. Rabbit anti-HA antibody and goat anti-rabbit IgG were used as the primary and secondary antibodies, respectively. B The relative copy number of genes encoding GCRV nonstructural protein NS80 and structural protein VP5 in cells transfected with different plasmids. C RT-qPCR confirm the knockdown of gapdhs by siRNAs. Cells were transfected with the corresponding siRNA for 24 h and harvested for RT-qPCR analysis. D The relative copy number of genes encoding NS80 and VP5 in cells transfected with different siRNAs. E RT-qPCR confirm the knockdown of ldha by siRNAs. F The relative copy number of genes encoding NS80 and VP5 in cells transfected with different siRNAs. Significant differences (P < 0.05) between the control and treated groups are indicated with asterisks (*)