Fig. 2

Most "hyper-long" telomere gut-associated leukocytes in zebrafish are macrophages. A Telo-FISH (red) combined with anti-RFP antibody to detect mpeg1.1:mcherry caax⁺ cells (green), which have been described as mostly macrophages and a subset of B-cells; anti-TCR (green) to detect T-cells and anti-GFP to detect mpx:GFP + expressing cells, which have been identified as neutrophils. Boxed areas show in yellow are expanded and displayed in the bottom panels. B mhcIIdab-GFP/cd45-DSRED transgenic line was used to perform Telo-FISH (red) combined with anti-GFP to detect mhcIIdab-GFP and anti-RFP to detect cd45-DSRED. Double mhcIIdab-GFP⁺/ cd45-DSRED⁺ have been identified as macrophages or dendritic cells; single mhcIIdab-GFP⁺ as B-cells and single cd45-DSRED as T-cells or neutrophils. Boxed areas show in yellow are expanded and displayed in the right panels. B1 Quantification of the relative proportion of immune sub-types within the “hyper-long” telomere population, using the transgenic from B). C Quantification of the % of “hyper-long” telomere cells that are mpeg.⁺, using the mpeg1.1:mcherry caax transgenic from A All animals are WT for telomerase, and are of a young age (c.5 months). Nuclei are counterstained with DAPI (blue) and all scale bars represent 50 µm, unless stated otherwise in the figure