Fig. 4

Fasting and postprandial monocyte counts and subsets. A Schematic representation of feeding regimen including standardized dinner meal and morning challenge meal with fasting and postprandial blood draws. Absolute numbers of peripheral blood monocytes at (B) fasting, 3, and 6 h after consumption of challenge meal for all study participants or (C) grouped by subject age. D classical, (E) intermediate, and (F) non-classical monocytes from all study subjects displayed as percentage of total monocytes. Mean and individual data points are shown. Statistical analyses in (A, B, D, E, and F) were performed using Friedman non-parametric, repeated measures one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant. Statistical analysis in C was performed using Wilcoxon paired, non-parametric, two-tailed t test; ***P < 0.001, ns = not significant