Fig. 4

Correlation of CD70 expression and cell division/expansion of CD8⁺ T_CM cells in vitro. A Gating strategy for CD70⁺ and CD70^- cells. Representative flow cytometry plots showing isolating CD70⁺ and CD70^- cells from CD28⁺ CD8⁺ T_CM cells using FACS sort. B CD70⁺ cells expanded better than CD70^- cells in vitro. Sorted CD70⁺ and CD70^- CD8⁺ T_CM cells were stimulated with anti-CD3/CD28 for 4 days (N=12). C Representative flow cytometry plots of cell division. Sorted CD70⁺ and CD70^- CD8⁺ T_CM cells were labeled with CTFR and stimulated with anti-CD3/CD28 for 4 days. Cell divisions were analyzed by Modfit, and replication index was presented (N=6). D CD70⁺ CD8⁺ T_CM cells had more cell divisions than CD70^- CD8⁺ T_CM cells as measured by the replication index (N=6). E Percentage of apoptotic cells in CD70⁺ and CD70^- CD8⁺ T_CM cells after 4 days of anti-CD3/CD28 stimulation (N=6). * Indicates P < 0.05, ** indicates P < 0.01