Fig. 1
Cisplatin induces tau deposits, and this is reversed by nasal administration of MSC. A Experimental timeline. Mice were treated with two rounds of 5 daily doses of cisplatin (Cis; 2.3 mg/kg/day i.p.) with 5 days of rest in between. Mesenchymal stem cells (MSC; 106 cells per dose) were administered intranasally at 48 and 96 hrs after completion of cisplatin treatment. Behavioral assessment was performed 45 days after MSC administration. Brains were collected for immunofluorescence and RT-PCR analysis at the time points indicated. B and C Representative images of tau immunostaining in coronal hippocampal (B) and entorhinal cortex (C) sections of 11.5-month-old female mice. (scale bar: 50 μm; Images taken using 20x objective/ 0.75 Numerical Aperture NA). D and E Quantification of the area of tau deposits in male (triangles) and female (circles) mice expressed as the percentage of total area. N = 7 females and 7 males per group. Data are represented as mean ± SEM and were analyzed by Two-way ANOVA with Tukey’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001. Three – way ANOVA revealed no statistically significant sex differences.