Fig. 3

Increased GFAP expression in astrocytes surrounding tau deposits. Representative images of the hippocampus from female mice treated with vehicle or cisplatin followed by nasal administration of MSC with double immunofluorescence labeling of tau-1 (green) and glial fibrillary acidic protein (GFAP; red) in the hippocampus at 40X (A) and 63X (B). Scale bar in (A) 50 μM and in (B) 25 μM. C High-resolution confocal imaging depicting individual and merged channels of the nuclear marker DAPI (blue), tau-1 (green) and GFAP (red) taken using 100x objective/1.45 NA. Scale bars 25 μm. The region of interest (ROI) in C imaged at higher magnification reveals (D) a single GFAP⁺ reactive astrocyte (red) with DAPI⁺ nucleus (blue), with its processes and tau (green) aggregates wrapping each other. Occasionally, tau-1 deposits were detected in the perinuclear region of the astrocyte. Image taken using 100x objective/1.45 NA and 4x scanner zoom. Scale bar 5 μm.