Fig. 5

Aquaporin 4 and tau colocalization. A Hippocampus and (B) entorhinal cortex of cisplatin-treated mouse showing multiple tau deposits (green) and their association with AQP4 (magenta). Images taken using 60x objective/1.4NA. Scale bars 25 μm. The tau deposits frequently occur in the disc-like nAQP4 domains in both (C) hippocampus and (D) entorhinal cortex. E Less frequently, tau deposits were detected in the tubular, perivascular endfeet (p-AQP4) of astrocytes with (F) 3D-orthogonal and (G) 3D-volume indicating their association with AQP4⁺ astrocytic endfeet and their nuclei. Images taken using 100x objective/1.45NA and 2x scanner zoom. Scale bars 5 μm.