Fig. 9

Experimental timeline for delayed treatment with MSC. A Male and female mice were treated with 2 rounds of cisplatin for 5 consecutive days and 5 days rest in between. MSC were administered intranasally at 31 and 33 days after cisplatin treatment completion. Cognition assessment was started 6 weeks after MSC administration. Tissue collection was done at the end of the experiment. Representative images of positive reactive immunostaining for tau in hippocampus (B) and entorhinal cortex EC (C) sections of 12.5-month-old female mice. Images taken using 20x objective/ 0.75 NA. Scale bars 50 μm. Quantification of images from hippocampus (D) and entorhinal cortex (E) of n = 8 mice (4 male, 4 female) per group. Data are represented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (Two-way ANOVA, Tukey’s post hoc test).