Fig. 4

APN-deprived aged mice displayed mitochondrial impairments. a: TMT-labeled proteomics. b: JC-1 aggregation ratio (n = 4–6). c-e: Representative bar graphs showing relative levels of ATP, MDA, and GSH in the experimental subjects (n = 4–6). f: Immunoblots and their quantitative column graphs, showing expression of NDUFA10, SDHB, UQCRFS1, ATP5A, Drp1, OPA1, PGC-1α, TFAM, LC3BI/II, and p62. Immunoblot densities were optimized by standard (GAPDH/β-actin) (n = 3). Data expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001